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Sarah W Harcum

TitleProfessor
InstitutionClemson University
DepartmentDepartment of Bioengineering
Address301 Rhodes Hall
Clemson, South Carolina 29634
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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Synoground BF, McGraw CE, Elliott KS, Leuze C, Roth JR, Harcum SW, Sandoval NR. Transient ammonia stress on Chinese hamster ovary (CHO) cells yield alterations to alanine metabolism and IgG glycosylation profiles. Biotechnol J. 2021 May 20; e2100098. PMID: 34014036.
      View in: PubMed
    2. Chitwood DG, Wang Q, Elliott K, Bullock A, Jordana D, Li Z, Wu C, Harcum SW, Saski CA. Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonia-stressed CHO cells grown in fed-batch cultures. BMC Biotechnol. 2021 Jan 08; 21(1):4. PMID: 33419422.
      View in: PubMed
    3. Odenwelder DC, Lu X, Harcum SW. Induced pluripotent stem cells can utilize lactate as a metabolic substrate to support proliferation. Biotechnol Prog. 2021 Mar; 37(2):e3090. PMID: 33029909.
      View in: PubMed
    4. Brodsky AN, Odenwelder DC, Harcum SW. High extracellular lactate causes reductive carboxylation in breast tissue cell lines grown under normoxic conditions. PLoS One. 2019; 14(6):e0213419. PMID: 31181081.
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    5. Gowtham YK, Saski CA, Harcum SW. Low glucose concentrations within typical industrial operating conditions have minimal effect on the transcriptome of recombinant CHO cells. Biotechnol Prog. 2017 May; 33(3):771-785. PMID: 28371311.
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    6. Wang J, Zhou J, Gowtham YK, Harcum SW, Husson SM. Antibody purification from CHO cell supernatant using new multimodal membranes. Biotechnol Prog. 2017 May; 33(3):658-665. PMID: 28248007.
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    7. Harcum SW, Lee KH. CHO Cells Can Make More Protein. Cell Syst. 2016 11 23; 3(5):412-413. PMID: 27883886.
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    8. Samoudi M, Minuchehr Z, Harcum SW, Tabandeh F, Omid Yeganeh N, Khodabandeh M. Rational design of glycoengineered interferon-ß analogs with improved aggregation state: experimental validation. Protein Eng Des Sel. 2017 Jan; 30(1):23-30. PMID: 27881683.
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    9. Brodsky AN, Caldwell M, Bae S, Harcum SW. Glycosylation-related genes in NS0 cells are insensitive to moderately elevated ammonium concentrations. J Biotechnol. 2014 Oct 10; 187:78-86. PMID: 25062658.
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    10. Baig F, Fernando LP, Salazar MA, Powell RR, Bruce TF, Harcum SW. Dynamic transcriptional response of Escherichia coli to inclusion body formation. Biotechnol Bioeng. 2014 May; 111(5):980-99. PMID: 24338599.
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    11. Gowtham YK, Miller KP, Hodge DB, Henson JM, Harcum SW. Novel two-stage fermentation process for bioethanol production using Saccharomyces pastorianus. Biotechnol Prog. 2014 Mar-Apr; 30(2):300-10. PMID: 24376155.
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    12. Wang L, Pepper ME, Padmakumar A, Burg TC, Harcum SW, Groff RE. A real-time adaptive oxygen transfer rate estimator for metabolism tracking in Escherichia coli cultures. Annu Int Conf IEEE Eng Med Biol Soc. 2014; 2014:6191-4. PMID: 25571411.
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    13. Pepper ME, Wang L, Padmakumar A, Burg TC, Harcum SW, Groff RE. A CMI (cell metabolic indicator)-based controller for achieving high growth rate Escherichia coli cultures. Annu Int Conf IEEE Eng Med Biol Soc. 2014; 2014:2911-5. PMID: 25570600.
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    14. Salazar MA, Fernando LP, Baig F, Harcum SW. The effects of protein solubility on the RNA Integrity Number (RIN) for recombinant Escherichia coli. Biochem Eng J. 2013 Oct 15; 79:129-135. PMID: 24151430.
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    15. Brodsky AN, Zhang J, Visconti RP, Harcum SW. Expansion of mesenchymal stem cells under atmospheric carbon dioxide. Biotechnol Prog. 2013 Sep-Oct; 29(5):1298-306. PMID: 23894049.
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    16. Miller KP, Gowtham YK, Henson JM, Harcum SW. Xylose isomerase improves growth and ethanol production rates from biomass sugars for both Saccharomyces pastorianus and Saccharomyces cerevisiae. Biotechnol Prog. 2012 May-Jun; 28(3):669-80. PMID: 22866331.
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    17. Wuest DM, Harcum SW, Lee KH. Genomics in mammalian cell culture bioprocessing. Biotechnol Adv. 2012 May-Jun; 30(3):629-38. PMID: 22079893.
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    18. Brodsky AN, Caldwell M, Harcum SW. Glycosylation and post-translational modification gene expression analysis by DNA microarrays for cultured mammalian cells. Methods. 2012 Mar; 56(3):408-17. PMID: 22033470.
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    19. Haddadin FT, Kurtz H, Harcum SW. Serine hydroxamate and the transcriptome of high cell density recombinant Escherichia coli MG1655. Appl Biochem Biotechnol. 2009 May; 157(2):124-39. PMID: 18612598.
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    20. Sharma SS, Campbell JW, Frisch D, Blattner FR, Harcum SW. Expression of two recombinant chloramphenicol acetyltransferase variants in highly reduced genome Escherichia coli strains. Biotechnol Bioeng. 2007 Dec 01; 98(5):1056-70. PMID: 17497738.
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    21. Chen P, Harcum SW. Differential display identifies genes in Chinese hamster ovary cells sensitive to elevated ammonium. Appl Biochem Biotechnol. 2007 May-Jun; 141(2-3):349-59. PMID: 18025561.
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    22. Sharma SS, Blattner FR, Harcum SW. Recombinant protein production in an Escherichia coli reduced genome strain. . 2007 Mar; 9(2):133-41. PMID: 17126054.
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    23. Harcum SW, Haddadin FT. Global transcriptome response of recombinant Escherichia coli to heat-shock and dual heat-shock recombinant protein induction. J Ind Microbiol Biotechnol. 2006 Oct; 33(10):801-14. PMID: 16680459.
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    24. Pósfai G, Plunkett G, Fehér T, Frisch D, Keil GM, Umenhoffer K, Kolisnychenko V, Stahl B, Sharma SS, de Arruda M, Burland V, Harcum SW, Blattner FR. Emergent properties of reduced-genome Escherichia coli. Science. 2006 May 19; 312(5776):1044-6. PMID: 16645050.
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    25. Sharma SS, Chong S, Harcum SW. Intein-mediated protein purification of fusion proteins expressed under high-cell density conditions in E. coli. J Biotechnol. 2006 Aug 20; 125(1):48-56. PMID: 16546284.
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    26. Chen P, Harcum SW. Effects of elevated ammonium on glycosylation gene expression in CHO cells. . 2006 Mar; 8(2):123-32. PMID: 16380282.
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    27. Sharma SS, Chong S, Harcum SW. Simulation of large-scale production of a soluble recombinant protein expressed in Escherichia coli using an intein-mediated purification system. Appl Biochem Biotechnol. 2005 Aug; 126(2):93-118. PMID: 16118465.
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    28. Clark KJ, Griffiths J, Bailey KM, Harcum SW. Gene-expression profiles for five key glycosylation genes for galactose-fed CHO cells expressing recombinant IL-4/13 cytokine trap. Biotechnol Bioeng. 2005 Jun 05; 90(5):568-77. PMID: 15818560.
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    29. Clark KJ, Harcum SW, Griffiths J, Bailey KM. Development of a method to quantify gene expression levels for glycosylation pathway genes in chinese hamster ovary cell cultures. Appl Biochem Biotechnol. 2005 Jun; 125(3):159-73. PMID: 15917580.
      View in: PubMed
    30. Haddadin FT, Harcum SW. Transcriptome profiles for high-cell-density recombinant and wild-type Escherichia coli. Biotechnol Bioeng. 2005 Apr 20; 90(2):127-53. PMID: 15742388.
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    31. Chen P, Harcum SW. Effects of amino acid additions on ammonium stressed CHO cells. J Biotechnol. 2005 May 25; 117(3):277-86. PMID: 15862358.
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    32. Clark KJ, Chaplin FW, Harcum SW. Temperature effects on product-quality-related enzymes in batch CHO cell cultures producing recombinant tPA. Biotechnol Prog. 2004 Nov-Dec; 20(6):1888-92. PMID: 15575729.
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    33. Sharma S, Zhang A, Wang H, Harcum SW, Chong S. Study of protein splicing and intein-mediated peptide bond cleavage under high-cell-density conditions. Biotechnol Prog. 2003 May-Jun; 19(3):1085-90. PMID: 12790686.
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    34. Harcum SW. Structured model to predict intracellular amino acid shortages during recombinant protein overexpression in E. coli. J Biotechnol. 2002 Feb 28; 93(3):189-202. PMID: 11755983.
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    35. Jordan GL, Harcum SW. Characterization of up-regulated proteases in an industrial recombinant Escherichia coli fermentation. J Ind Microbiol Biotechnol. 2002 Feb; 28(2):74-80. PMID: 12074055.
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    36. Harcum SW, Bentley WE. Heat-shock and stringent responses have overlapping protease activity in Escherichia coli. Implications for heterologous protein yield. Appl Biochem Biotechnol. 1999 Apr; 80(1):23-37. PMID: 10394618.
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    37. Harcum SW, Bentley WE. Response dynamics of 26-, 34-, 39-, 54-, and 80-kDa proteases in induced cultures of recombinant Escherichia coli. Biotechnol Bioeng. 1993 Sep 05; 42(6):675-85. PMID: 18613100.
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