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Zsolt Ablonczy

Title
InstitutionMedical University of South Carolina
DepartmentOphthalmology
AddressP.O. Box MSC 676
518A
167 Ashley Ave.
Phone843-792-0777
Fax843-792-1723
Email
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    Collapse Biography 
    Collapse awards and honors
    1997Travel Award, Agricultural University of Wroclaw, Wroclaw, Poland
    1997Travel Award, French Ministry of Foreign Affairs, Paris, France
    1997Travel Award, Ruder Boškovic Institute, Zagreb, Croatia
    2000Travel Award, American Society for Photobiology
    2000Travel Award, Hungarian Ministry of Education, Budapest, Hungary
    2001Cover Article, Analytical Chemistry
    2004Travel Award, International Congress on Retinal Proteins
    2005Paper of the Week, Journal of Biological Chemistry
    2010Travel Award, International Congress on Retinal Proteins
    2011Cover Article, Investigative Ophthalmology and Visual Science
    2013Invited Participant of the Audacious Goals Meeting, National Eye Institute

    Collapse Overview 
    Collapse overview
    My laboratory is interested in understanding how endogenous and environmental factors influence the function of the retinal pigment epithelium (RPE) and contribute to the development and resolution of degenerative retinal diseases. Our research focuses on two main areas, diabetic retinal edema and age-related macular degeneration.

    Retinal edema, the accumulation of fluid within the extracellular spaces of the neurosensory retina, is a common element in several retinal diseases and is a leading cause of vision loss in the Western countries. While the principle source of this extracellular fluid is considered to be leakage from blood vessels, our studies have demonstrated that alterations in RPE function also contribute to the development of retinal edema. Pathologic conditions, such as hyperglycemia or the accumulation of advanced glycation-end products in the vitreous fluid induce the secretion of cytokines. We have previously shown that these cytokines can alter the barrier function and secretory properties of the RPE. Our recent studies in vivo and in vitro provided new evidence that natriuretic peptides (NPs), hormones with diuretic and vasodilatory properties, have the ability to prevent the cytokine-induced breakdown of RPE fluid resorption and barrier function. Therefore, we expect that are likely to be a beneficial for edematous fluid and a convenient addition to the current limited spectrum of clinical treatment options.

    Age-related macular degeneration (AMD) is responsible for most old-age visual loss in the United States. A potentially crucial factor in the development of AMD is the fluorescent lipofuscin, which accumulates with age in the lysosomal compartment of the RPE and primarily in the posterior pole of the eye. A principal component of lipofuscin is the vitamin-A by-product, A2E, which is toxic at high concentrations in cell culture models. However, due to a mismatch between the traditional methods of detection for lipofuscin (fluorescence) and A2E (bioanalytics), it has not been previously possible to directly correlate A2E with lipofuscin. Utilizing imaging mass spectrometry (MALDI-IMS), we have recently managed to bridge this gap and to determine the spatial distributions of A2E and lipofuscin in the human RPE. Our data provided convincing evidence that lipofuscin and A2E do not spatially correlate in the human eye (independent of AMD condition). Therefore, my laboratory is engaged in understanding the toxic component of lipofuscin, and the significance of A2E in the periphery. MALDI-IMS provides a unique opportunity to chemically identify relevant molecular information based on imaging modalities commonly used for clinical diagnosis (such as fluorescence).


    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Mohammed T, Tong Y, Agee J, Challa N, Heintzmann R, Hammer M, Curcio CA, Ach T, Ablonczy Z, Smith RT. Ex Vivo Hyperspectral Autofluorescence Imaging and Localization of Fluorophores in Human Eyes with Age-Related Macular Degeneration. Vision (Basel). 2018 Sep 26; 2(4). PMID: 31735901.
      View in: PubMed
    2. Shah N, Ishii M, Brandon C, Ablonczy Z, Cai J, Liu Y, Chou CJ, Rohrer B. Extracellular vesicle-mediated long-range communication in stressed retinal pigment epithelial cell monolayers. Biochim Biophys Acta Mol Basis Dis. 2018 Aug; 1864(8):2610-2622. PMID: 29684588.
      View in: PubMed
    3. Anderson DMG, Ablonczy Z, Koutalos Y, Hanneken AM, Spraggins JM, Calcutt MW, Crouch RK, Caprioli RM, Schey KL. Bis(monoacylglycero)phosphate lipids in the retinal pigment epithelium implicate lysosomal/endosomal dysfunction in a model of Stargardt disease and human retinas. Sci Rep. 2017 12 11; 7(1):17352. PMID: 29229934.
      View in: PubMed
    4. Anderson DMG, Lambert W, Calkins DJ, Ablonczy Z, Crouch RK, Caprioli RM, Schey KL. Imaging MS of Rodent Ocular Tissues and the Optic Nerve. Methods Mol Biol. 2017; 1618:15-27. PMID: 28523496.
      View in: PubMed
    5. Tong Y, Ben Ami T, Hong S, Heintzmann R, Gerig G, Ablonczy Z, Curcio CA, Ach T, Smith RT. HYPERSPECTRAL AUTOFLUORESCENCE IMAGING OF DRUSEN AND RETINAL PIGMENT EPITHELIUM IN DONOR EYES WITH AGE-RELATED MACULAR DEGENERATION. Retina. 2016 Dec; 36 Suppl 1:S127-S136. PMID: 28005671.
      View in: PubMed
    6. Desjardins D, Liu Y, Crosson CE, Ablonczy Z. Histone Deacetylase Inhibition Restores Retinal Pigment Epithelium Function in Hyperglycemia. PLoS One. 2016; 11(9):e0162596. PMID: 27617745.
      View in: PubMed
    7. Ben Ami T, Tong Y, Bhuiyan A, Huisingh C, Ablonczy Z, Ach T, Curcio CA, Smith RT. Spatial and Spectral Characterization of Human Retinal Pigment Epithelium Fluorophore Families by Ex Vivo Hyperspectral Autofluorescence Imaging. Transl Vis Sci Technol. 2016 May; 5(3):5. PMID: 27226929.
      View in: PubMed
    8. Desjardins DM, Yates PW, Dahrouj M, Liu Y, Crosson CE, Ablonczy Z. Progressive Early Breakdown of Retinal Pigment Epithelium Function in Hyperglycemic Rats. Invest Ophthalmol Vis Sci. 2016 05 01; 57(6):2706-13. PMID: 27191823.
      View in: PubMed
    9. Bowrey HE, Anderson DM, Pallitto P, Gutierrez DB, Fan J, Crouch RK, Schey KL, Ablonczy Z. Imaging mass spectrometry of the visual system: Advancing the molecular understanding of retina degenerations. Proteomics Clin Appl. 2016 Apr; 10(4):391-402. PMID: 26586164.
      View in: PubMed
    10. Starnes AC, Huisingh C, McGwin G, Sloan KR, Ablonczy Z, Smith RT, Curcio CA, Ach T. Multi-nucleate retinal pigment epithelium cells of the human macula exhibit a characteristic and highly specific distribution. Vis Neurosci. 2016; 33:e001. PMID: 26923500.
      View in: PubMed
    11. Gong J, Fields MA, Moreira EF, Bowrey HE, Gooz M, Ablonczy Z, Del Priore LV. Differentiation of Human Protein-Induced Pluripotent Stem Cells toward a Retinal Pigment Epithelial Cell Fate. PLoS One. 2015; 10(11):e0143272. PMID: 26606685.
      View in: PubMed
    12. Adler L, Boyer NP, Anderson DM, Spraggins JM, Schey KL, Hanneken A, Ablonczy Z, Crouch RK, Koutalos Y. Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium. Photochem Photobiol Sci. 2015 Nov; 14(11):1983-90. PMID: 26323192.
      View in: PubMed
    13. Pallitto P, Ablonczy Z, Jones EE, Drake RR, Koutalos Y, Crouch RK, Donello J, Herrmann J. A2E and lipofuscin distributions in macaque retinal pigment epithelium are similar to human. Photochem Photobiol Sci. 2015 Oct; 14(10):1888-95. PMID: 26223373.
      View in: PubMed
    14. Crouch RK, Koutalos Y, Kono M, Schey K, Ablonczy Z. A2E and Lipofuscin. Prog Mol Biol Transl Sci. 2015; 134:449-63. PMID: 26310170.
      View in: PubMed
    15. Fields MA, Bowrey HE, Gong J, Ablonczy Z, Del Priore LV. Retinoid Processing in Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelium Cultures. Prog Mol Biol Transl Sci. 2015; 134:477-90. PMID: 26310172.
      View in: PubMed
    16. Dahrouj M, Desjardins DM, Liu Y, Crosson CE, Ablonczy Z. Receptor mediated disruption of retinal pigment epithelium function in acute glycated-albumin exposure. Exp Eye Res. 2015 Aug; 137:50-6. PMID: 26070987.
      View in: PubMed
    17. Adler L, Boyer NP, Chen C, Ablonczy Z, Crouch RK, Koutalos Y. The 11-cis Retinal Origins of Lipofuscin in the Retina. Prog Mol Biol Transl Sci. 2015; 134:e1-12. PMID: 26310175.
      View in: PubMed
    18. Smith RT, Post R, Johri A, Lee MD, Ablonczy Z, Curcio CA, Ach T, Sajda P. Simultaneous decomposition of multiple hyperspectral data sets: signal recovery of unknown fluorophores in the retinal pigment epithelium. Biomed Opt Express. 2014 Dec 01; 5(12):4171-85. PMID: 25574430.
      View in: PubMed
    19. Ach T, Huisingh C, McGwin G, Messinger JD, Zhang T, Bentley MJ, Gutierrez DB, Ablonczy Z, Smith RT, Sloan KR, Curcio CA. Quantitative autofluorescence and cell density maps of the human retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2014 Jul 17; 55(8):4832-41. PMID: 25034602.
      View in: PubMed
    20. Sun D, Nakao S, Xie F, Zandi S, Bagheri A, Kanavi MR, Samiei S, Soheili ZS, Frimmel S, Zhang Z, Ablonczy Z, Ahmadieh H, Hafezi-Moghadam A. Molecular imaging reveals elevated VEGFR-2 expression in retinal capillaries in diabetes: a novel biomarker for early diagnosis. FASEB J. 2014 Sep; 28(9):3942-51. PMID: 24903276.
      View in: PubMed
    21. Anderson DM, Ablonczy Z, Koutalos Y, Spraggins J, Crouch RK, Caprioli RM, Schey KL. High resolution MALDI imaging mass spectrometry of retinal tissue lipids. J Am Soc Mass Spectrom. 2014 Aug; 25(8):1394-403. PMID: 24819461.
      View in: PubMed
    22. Dahrouj M, Alsarraf O, McMillin JC, Liu Y, Crosson CE, Ablonczy Z. Vascular endothelial growth factor modulates the function of the retinal pigment epithelium in vivo. Invest Ophthalmol Vis Sci. 2014 Apr 09; 55(4):2269-75. PMID: 24550368.
      View in: PubMed
    23. Barnwell E, Padmaraju V, Baranello R, Pacheco-Quinto J, Crosson C, Ablonczy Z, Eckman E, Eckman CB, Ramakrishnan V, Greig NH, Pappolla MA, Sambamurti K. Evidence of a novel mechanism for partial ?-secretase inhibition induced paradoxical increase in secreted amyloid ß protein. PLoS One. 2014; 9(3):e91531. PMID: 24658363.
      View in: PubMed
    24. Ablonczy Z, Smith N, Anderson DM, Grey AC, Spraggins J, Koutalos Y, Schey KL, Crouch RK. The utilization of fluorescence to identify the components of lipofuscin by imaging mass spectrometry. Proteomics. 2014 Apr; 14(7-8):936-44. PMID: 24453194.
      View in: PubMed
    25. Ablonczy Z, Dahrouj M, Marneros AG. Progressive dysfunction of the retinal pigment epithelium and retina due to increased VEGF-A levels. FASEB J. 2014 May; 28(5):2369-79. PMID: 24558195.
      View in: PubMed
    26. Ablonczy Z, Higbee D, Grey AC, Koutalos Y, Schey KL, Crouch RK. Similar molecules spatially correlate with lipofuscin and N-retinylidene-N-retinylethanolamine in the mouse but not in the human retinal pigment epithelium. Arch Biochem Biophys. 2013 Nov 15; 539(2):196-202. PMID: 23969078.
      View in: PubMed
    27. Ablonczy Z, Higbee D, Anderson DM, Dahrouj M, Grey AC, Gutierrez D, Koutalos Y, Schey KL, Hanneken A, Crouch RK. Lack of correlation between the spatial distribution of A2E and lipofuscin fluorescence in the human retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2013 Aug 15; 54(8):5535-42. PMID: 23847313.
      View in: PubMed
    28. Kim JY, Zhao H, Martinez J, Doggett TA, Kolesnikov AV, Tang PH, Ablonczy Z, Chan CC, Zhou Z, Green DR, Ferguson TA. Noncanonical autophagy promotes the visual cycle. Cell. 2013 Jul 18; 154(2):365-76. PMID: 23870125.
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    29. Dahrouj M, Alsarraf O, Liu Y, Crosson CE, Ablonczy Z. C-type natriuretic peptide protects the retinal pigment epithelium against advanced glycation end product-induced barrier dysfunction. J Pharmacol Exp Ther. 2013 Jan; 344(1):96-102. PMID: 23086231.
      View in: PubMed
    30. Tang PH, Kono M, Koutalos Y, Ablonczy Z, Crouch RK. New insights into retinoid metabolism and cycling within the retina. Prog Retin Eye Res. 2013 Jan; 32:48-63. PMID: 23063666.
      View in: PubMed
    31. Boyer NP, Higbee D, Currin MB, Blakeley LR, Chen C, Ablonczy Z, Crouch RK, Koutalos Y. Lipofuscin and N-retinylidene-N-retinylethanolamine (A2E) accumulate in retinal pigment epithelium in absence of light exposure: their origin is 11-cis-retinal. J Biol Chem. 2012 Jun 22; 287(26):22276-86. PMID: 22570475.
      View in: PubMed
    32. Boyer NP, Tang PH, Higbee D, Ablonczy Z, Crouch RK, Koutalos Y. Lipofuscin and A2E accumulate with age in the retinal pigment epithelium of Nrl-/- mice. Photochem Photobiol. 2012 Nov-Dec; 88(6):1373-7. PMID: 22417141.
      View in: PubMed
    33. Ablonczy Z, Gutierrez DB, Grey AC, Schey KL, Crouch RK. Molecule-specific imaging and quantitation of A2E in the RPE. Adv Exp Med Biol. 2012; 723:75-81. PMID: 22183318.
      View in: PubMed
    34. Ablonczy Z, Dahrouj M, Tang PH, Liu Y, Sambamurti K, Marmorstein AD, Crosson CE. Human retinal pigment epithelium cells as functional models for the RPE in vivo. Invest Ophthalmol Vis Sci. 2011 Nov 04; 52(12):8614-20. PMID: 21960553.
      View in: PubMed
    35. Grey AC, Crouch RK, Koutalos Y, Schey KL, Ablonczy Z. Spatial localization of A2E in the retinal pigment epithelium. Invest Ophthalmol Vis Sci. 2011 Jun 06; 52(7):3926-33. PMID: 21357388.
      View in: PubMed
    36. Truscott RJ, Comte-Walters S, Ablonczy Z, Schwacke JH, Berry Y, Korlimbinis A, Friedrich MG, Schey KL. Tight binding of proteins to membranes from older human cells. Age (Dordr). 2011 Dec; 33(4):543-54. PMID: 21181282.
      View in: PubMed
    37. Gutierrez DB, Blakeley L, Goletz PW, Schey KL, Hanneken A, Koutalos Y, Crouch RK, Ablonczy Z. Mass spectrometry provides accurate and sensitive quantitation of A2E. Photochem Photobiol Sci. 2010 Nov; 9(11):1513-9. PMID: 20931136.
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    38. Chinnakkannu P, Samanna V, Cheng G, Ablonczy Z, Baicu CF, Bethard JR, Menick DR, Kuppuswamy D, Cooper G. Site-specific microtubule-associated protein 4 dephosphorylation causes microtubule network densification in pressure overload cardiac hypertrophy. J Biol Chem. 2010 Jul 09; 285(28):21837-48. PMID: 20436166.
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    39. Prakasam A, Muthuswamy A, Ablonczy Z, Greig NH, Fauq A, Rao KJ, Pappolla MA, Sambamurti K. Differential accumulation of secreted AbetaPP metabolites in ocular fluids. J Alzheimers Dis. 2010; 20(4):1243-53. PMID: 20413851.
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    40. Lara-Castillo N, Zandi S, Nakao S, Ito Y, Noda K, She H, Ahmed M, Frimmel S, Ablonczy Z, Hafezi-Moghadam A. Atrial natriuretic peptide reduces vascular leakage and choroidal neovascularization. Am J Pathol. 2009 Dec; 175(6):2343-50. PMID: 19910509.
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    41. Ablonczy Z, Prakasam A, Fant J, Fauq A, Crosson C, Sambamurti K. Pigment epithelium-derived factor maintains retinal pigment epithelium function by inhibiting vascular endothelial growth factor-R2 signaling through gamma-secretase. J Biol Chem. 2009 Oct 30; 284(44):30177-86. PMID: 19723623.
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    42. Thurman JM, Renner B, Kunchithapautham K, Ferreira VP, Pangburn MK, Ablonczy Z, Tomlinson S, Holers VM, Rohrer B. Oxidative stress renders retinal pigment epithelial cells susceptible to complement-mediated injury. J Biol Chem. 2009 Jun 19; 284(25):16939-47. PMID: 19386604.
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    43. Takahashi Y, Moiseyev G, Ablonczy Z, Chen Y, Crouch RK, Ma JX. Identification of a novel palmitylation site essential for membrane association and isomerohydrolase activity of RPE65. J Biol Chem. 2009 Jan 30; 284(5):3211-8. PMID: 19049981.
      View in: PubMed
    44. Jackson W, Ablonczy Z, Crouch RK. Quantitation of the effect of hydroxylamine on rhodopsin palmitylation. Photochem Photobiol. 2008 Jul-Aug; 84(4):949-55. PMID: 18399918.
      View in: PubMed
    45. Ablonczy Z, Crosson CE. VEGF modulation of retinal pigment epithelium resistance. Exp Eye Res. 2007 Dec; 85(6):762-71. PMID: 17915218.
      View in: PubMed
    46. Ablonczy Z, Kono M, Knapp DR, Crouch RK. Palmitylation of cone opsins. Vision Res. 2006 Dec; 46(27):4493-501. PMID: 16989884.
      View in: PubMed
    47. Ablonczy Z, Darrow RM, Knapp DR, Organisciak DT, Crouch RK. Rhodopsin phosphorylation in rats exposed to intense light. Photochem Photobiol. 2005 May-Jun; 81(3):541-7. PMID: 15504085.
      View in: PubMed
    48. Wang Z, Wen XH, Ablonczy Z, Crouch RK, Makino CL, Lem J. Enhanced shutoff of phototransduction in transgenic mice expressing palmitoylation-deficient rhodopsin. J Biol Chem. 2005 Jul 01; 280(26):24293-300. PMID: 15851469.
      View in: PubMed
    49. Ablonczy Z, Crouch RK, Knapp DR. Mass spectrometric analysis of integral membrane proteins at the subpicomolar level: application to rhodopsin. J Chromatogr B Analyt Technol Biomed Life Sci. 2005 Oct 25; 825(2):169-75. PMID: 16213448.
      View in: PubMed
    50. Wang X, Kim SH, Ablonczy Z, Crouch RK, Knapp DR. Probing rhodopsin-transducin interactions by surface modification and mass spectrometry. Biochemistry. 2004 Sep 07; 43(35):11153-62. PMID: 15366925.
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    51. Ablonczy Z, Lukács A, Papp E. Application of the maximum entropy method to absorption kinetic rate processes. Biophys Chem. 2003 May 01; 104(1):249-58. PMID: 12834843.
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    52. Rohrer B, Goletz P, Znoiko S, Ablonczy Z, Ma JX, Redmond TM, Crouch RK. Correlation of regenerable opsin with rod ERG signal in Rpe65-/- mice during development and aging. Invest Ophthalmol Vis Sci. 2003 Jan; 44(1):310-5. PMID: 12506090.
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    53. Rohrer B, Ablonczy Z, Znoiko S, Redmond M, Ma JX, Crouch R. Does constitutive phosphorylation protect against photoreceptor degeneration in Rpe65-/- mice? Adv Exp Med Biol. 2003; 533:221-7. PMID: 15180268.
      View in: PubMed
    54. Ablonczy Z, Crouch RK, Goletz PW, Redmond TM, Knapp DR, Ma JX, Rohrer B. 11-cis-retinal reduces constitutive opsin phosphorylation and improves quantum catch in retinoid-deficient mouse rod photoreceptors. J Biol Chem. 2002 Oct 25; 277(43):40491-8. PMID: 12176991.
      View in: PubMed
    55. Ablonczy Z, Goletz P, Knapp DR, Crouch RK. Mass spectrometric analysis of porcine rhodopsin. Photochem Photobiol. 2002 Mar; 75(3):316-21. PMID: 11950099.
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    56. Knapp DR, Crouch RK, Ball LE, Gelasco AK, Ablonczy Z. Mass spectrometric analysis of G protein-coupled receptors. Methods Enzymol. 2002; 343:157-61. PMID: 11665563.
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    57. Imasheva ES, Lu M, Balashov SP, Ebrey TG, Chen Y, Ablonczy Z, Menick DR, Crouch RK. Exploring the function of Tyr83 in bacteriorhodopsin: features of the Y83F and Y83N mutants. Biochemistry. 2001 Nov 06; 40(44):13320-30. PMID: 11683642.
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    58. Ablonczy Z, Kono M, Crouch RK, Knapp DR. Mass spectrometric analysis of integral membrane proteins at the subnanomolar level: application to recombinant photopigments. Anal Chem. 2001 Oct 15; 73(20):4774-9. PMID: 11681450.
      View in: PubMed
    59. Ma J, Zhang J, Othersen KL, Moiseyev G, Ablonczy Z, Redmond TM, Chen Y, Crouch RK. Expression, purification, and MALDI analysis of RPE65. Invest Ophthalmol Vis Sci. 2001 Jun; 42(7):1429-35. PMID: 11381042.
      View in: PubMed
    60. Ablonczy Z, Knapp DR, Darrow R, Organisciak DT, Crouch RK. Mass spectrometric analysis of rhodopsin from light damaged rats. Mol Vis. 2000 Jun 27; 6:109-15. PMID: 10874059.
      View in: PubMed
    61. Ablonczy Z, Ha VH, Papp E. Actinic light and pH effect on the proton pumping of bacteriorhodopsin. Biophys Chem. 1998 Apr 20; 71(2-3):235-43. PMID: 17029699.
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