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Last Name
Institution

John E. Baatz

Title
InstitutionMedical University of South Carolina
DepartmentPediatrics
AddressP.O. Box MSC 514
CR 307
173 Ashley Ave.
Phone843-792-1049
Fax843-792-1844
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    The focus of the research projects in our laboratory is the effects of hypoxic stress and marine-borne toxin exposure on the distal lung, specifically with respect to responses of alveolar cells. This research currently encompasses four research projects. The first of these examines the roles of hemoglobin, recently identified by our laboratory as being expressed in alveolar type II cells, in nitrosative and oxidative stress as well as surfactant protein expression. Secondly we are developing a porous hollow fiber system that permits culture of embryonic stem cells, primary airway cells and immortalized airway cells within the fiber at an air/water interface. We are also using this system to assess adaptations diving marine mammals (such as dolphins, seals and whales) have made to repetitive hypoxic conditions while being able to maintain lung health. This unique technique may be useful in reducing the use of animals in studying distal lung during health, disease or injury, and may also serve as a system to repopulate diseased or injured airway cells with health cells. We continue to examine structural/function relationships of pulmonary surfactant proteins and the effects of marine-borne toxins (such as red-tide toxins) on lung health. This is an exciting untapped field of study and has direct impacts on human lung health since humans share the often-polluted coastal waters, whether by recreation or work related, with dolphins, whales and manatees. This project utilizes proteomic and molecular biologic techniques in an effort to identify proteins and specific mechanistic pathways in airway cells that are altered in expression or secretion as a result of toxin exposure. Finally, as Director of the MUSC Gel Proteomics facility, I am interested in development of novel separations and labeling techniques to identify, in a variety of species, disease-specific protein biomarkers and effects of drugs or stress on subcellular organelle proteins.

    Collapse keywords
    Neonatal lung diseases, Oxygen homeostasis and lung disease, Effects of oxygen on lung development, Lung cryopreservation

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    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Newton DA, Baatz JE, Kindy MS, Gattoni-Celli S, Shary JR, Hollis BW, Wagner CL. Insights image for vitamin D binding protein polymorphisms significantly impact vitamin D status in children. Pediatr Res. 2019 Jun 24. PMID: 31234192.
      View in: PubMed
    2. Temkin AM, Bowers RR, Ulmer CZ, Penta K, Bowden JA, Nyland J, Baatz JE, Spyropoulos DD. Increased adiposity, inflammation, metabolic disruption and dyslipidemia in adult male offspring of DOSS treated C57BL/6 dams. Sci Rep. 2019 Feb 06; 9(1):1530. PMID: 30728429.
      View in: PubMed
    3. Newton DA, Baatz JE, Kindy MS, Gattoni-Celli S, Shary JR, Hollis BW, Wagner CL. Vitamin D binding protein polymorphisms significantly impact vitamin D status in children. Pediatr Res. 2019 Feb 02. PMID: 30712059.
      View in: PubMed
    4. Wagner CL, Baatz JE, Newton D, Hollis BW. Analytical considerations and general diagnostic and therapeutic ramifications of milk hormones during lactation. Best Pract Res Clin Endocrinol Metab. 2018 01; 32(1):5-16. PMID: 29549960.
      View in: PubMed
    5. Ghatak S, Markwald RR, Hascall VC, Dowling W, Lottes RG, Baatz JE, Beeson G, Beeson CC, Perrella MA, Thannickal VJ, Misra S. Transforming growth factor ß1 (TGFß1) regulates CD44V6 expression and activity through extracellular signal-regulated kinase (ERK)-induced EGR1 in pulmonary fibrogenic fibroblasts. J Biol Chem. 2017 06 23; 292(25):10465-10489. PMID: 28389562.
      View in: PubMed
    6. Bowers RR, Temkin AM, Guillette LJ, Baatz JE, Spyropoulos DD. The commonly used nonionic surfactant Span 80 has RXRa transactivation activity, which likely increases the obesogenic potential of oil dispersants and food emulsifiers. Gen Comp Endocrinol. 2016 11 01; 238:61-68. PMID: 27131391.
      View in: PubMed
    7. Piroli GG, Manuel AM, Clapper AC, Walla MD, Baatz JE, Palmiter RD, Quintana A, Frizzell N. Succination is Increased on Select Proteins in the Brainstem of the NADH dehydrogenase (ubiquinone) Fe-S protein 4 (Ndufs4) Knockout Mouse, a Model of Leigh Syndrome. Mol Cell Proteomics. 2016 Feb; 15(2):445-61. PMID: 26450614.
      View in: PubMed
    8. Temkin AM, Bowers RR, Magaletta ME, Holshouser S, Maggi A, Ciana P, Guillette LJ, Bowden JA, Kucklick JR, Baatz JE, Spyropoulos DD. Effects of Crude Oil/Dispersant Mixture and Dispersant Components on PPAR? Activity in Vitro and in Vivo: Identification of Dioctyl Sodium Sulfosuccinate (DOSS; CAS #577-11-7) as a Probable Obesogen. Environ Health Perspect. 2016 Jan; 124(1):112-9. PMID: 26135921.
      View in: PubMed
    9. Lottes RG, Newton DA, Spyropoulos DD, Baatz JE. Lactate as substrate for mitochondrial respiration in alveolar epithelial type II cells. Am J Physiol Lung Cell Mol Physiol. 2015 May 01; 308(9):L953-61. PMID: 25747963.
      View in: PubMed
    10. Mancia A, Abelli L, Kucklick JR, Rowles TK, Wells RS, Balmer BC, Hohn AA, Baatz JE, Ryan JC. Microarray applications to understand the impact of exposure to environmental contaminants in wild dolphins (Tursiops truncatus). Mar Genomics. 2015 Feb; 19:47-57. PMID: 25479946.
      View in: PubMed
    11. Baatz JE, Newton DA, Riemer EC, Denlinger CE, Jones EE, Drake RR, Spyropoulos DD. Cryopreservation of viable human lung tissue for versatile post-thaw analyses and culture. In Vivo. 2014 Jul-Aug; 28(4):411-23. PMID: 24982205.
      View in: PubMed
    12. Lottes RG, Newton DA, Spyropoulos DD, Baatz JE. Alveolar type II cells maintain bioenergetic homeostasis in hypoxia through metabolic and molecular adaptation. Am J Physiol Lung Cell Mol Physiol. 2014 May 15; 306(10):L947-55. PMID: 24682450.
      View in: PubMed
    13. Glasser SW, Maxfield MD, Ruetschilling TL, Akinbi HT, Baatz JE, Kitzmiller JA, Page K, Xu Y, Bao EL, Korfhagen TR. Persistence of LPS-induced lung inflammation in surfactant protein-C-deficient mice. Am J Respir Cell Mol Biol. 2013 Nov; 49(5):845-54. PMID: 23795648.
      View in: PubMed
    14. Glasser SW, Senft AP, Maxfield MD, Ruetschilling TL, Baatz JE, Page K, Korfhagen TR. Genetic replacement of surfactant protein-C reduces respiratory syncytial virus induced lung injury. Respir Res. 2013 Feb 12; 14:19. PMID: 23399055.
      View in: PubMed
    15. Robinson CJ, Wagner CL, Hollis BW, Baatz JE, Johnson DD. Association of maternal vitamin D and placenta growth factor with the diagnosis of early onset severe preeclampsia. Am J Perinatol. 2013 Mar; 30(3):167-72. PMID: 22875657.
      View in: PubMed
    16. Mancia A, Elliott JT, Halter M, Bhadriraju K, Tona A, Spurlin TA, Middlebrooks BL, Baatz JE, Warr GW, Plant AL. Quantitative methods to characterize morphological properties of cell lines. Biotechnol Prog. 2012 Jul; 28(4):1069-78. PMID: 22619183.
      View in: PubMed
    17. Robinson C, Wagner C, Baatz J, Hollis B, Johnson D. OS068. 25-Hydroxyvitamin D and angiogenic factors in early-onset severe preeclampsia. Pregnancy Hypertens. 2012 Jul; 2(3):214. PMID: 26105282.
      View in: PubMed
    18. Robinson C, Wagner C, Hollis B, Baatz J, Johnson D. PP161. Association of maternal vitamin D and placenta growth factor with early-onset severe preeclampsia. Pregnancy Hypertens. 2012 Jul; 2(3):326. PMID: 26105482.
      View in: PubMed
    19. Unal ER, Lynn T, Neidich J, Salazar D, Goetzl L, Baatz JE, Hulsey TC, Van Dolah R, Guillette LJ, Newman R. Racial disparity in maternal and fetal-cord bisphenol A concentrations. J Perinatol. 2012 Nov; 32(11):844-50. PMID: 22402483.
      View in: PubMed
    20. Cryopreservation and in vitro culture of primary cell types from lung tissue of a stranded pygmy sperm whale (Kogia breviceps). Comp Biochem Physiol C Toxicol Pharmacol. 2012 Jan; 155(1):136-42. PMID: 21501697.
      View in: PubMed
    21. Robinson CJ, Wagner CL, Hollis BW, Baatz JE, Johnson DD. Maternal vitamin D and fetal growth in early-onset severe preeclampsia. Am J Obstet Gynecol. 2011 Jun; 204(6):556.e1-4. PMID: 21507371.
      View in: PubMed
    22. Sas KM, Baatz JE. Brevetoxin-2 induces an inflammatory response in an alveolar macrophage cell line. Int J Hyg Environ Health. 2010 Sep; 213(5):352-8. PMID: 20655277.
      View in: PubMed
    23. Grek CL, Newton DA, Spyropoulos DD, Baatz JE. Hypoxia up-regulates expression of hemoglobin in alveolar epithelial cells. Am J Respir Cell Mol Biol. 2011 Apr; 44(4):439-47. PMID: 20508070.
      View in: PubMed
    24. Demarse NA, Ponnusamy S, Spicer EK, Apohan E, Baatz JE, Ogretmen B, Davies C. Direct binding of glyceraldehyde 3-phosphate dehydrogenase to telomeric DNA protects telomeres against chemotherapy-induced rapid degradation. J Mol Biol. 2009 Dec 11; 394(4):789-803. PMID: 19800890.
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    25. Glasser SW, Witt TL, Senft AP, Baatz JE, Folger D, Maxfield MD, Akinbi HT, Newton DA, Prows DR, Korfhagen TR. Surfactant protein C-deficient mice are susceptible to respiratory syncytial virus infection. Am J Physiol Lung Cell Mol Physiol. 2009 Jul; 297(1):L64-72. PMID: 19304906.
      View in: PubMed
    26. Grek CL, Newton DA, Qiu Y, Wen X, Spyropoulos DD, Baatz JE. Characterization of alveolar epithelial cells cultured in semipermeable hollow fibers. Exp Lung Res. 2009 Mar; 35(2):155-74. PMID: 19263283.
      View in: PubMed
    27. Shockey JE, O'Leary NA, de la Vega E, Browdy CL, Baatz JE, Gross PS. The role of crustins in Litopenaeus vannamei in response to infection with shrimp pathogens: An in vivo approach. Dev Comp Immunol. 2009 May; 33(5):668-73. PMID: 19100764.
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    28. Yu H, Buff SM, Baatz JE, Virella-Lowell I. Oral instillation with surfactant phospholipid: a reliable alternative to intratracheal injection in mouse studies. Lab Anim. 2008 Jul; 42(3):294-304. PMID: 18625584.
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    29. Sinha R, Pinto JT, Facompre N, Kilheffer J, Baatz JE, El-Bayoumy K. Effects of naturally occurring and synthetic organoselenium compounds on protein profiling in androgen responsive and androgen independent human prostate cancer cells. Nutr Cancer. 2008; 60(2):267-75. PMID: 18444160.
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    30. Skinner ML, Schlosser RJ, Lathers D, Neal JG, Woodworth BA, Hall J, Newton DA, Baatz JE. Innate and adaptive mediators in cystic fibrosis and allergic fungal rhinosinusitis. Am J Rhinol. 2007 Sep-Oct; 21(5):538-41. PMID: 17999785.
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    31. Nagai R, Brock JW, Blatnik M, Baatz JE, Bethard J, Walla MD, Thorpe SR, Baynes JW, Frizzell N. Succination of protein thiols during adipocyte maturation: a biomarker of mitochondrial stress. J Biol Chem. 2007 Nov 23; 282(47):34219-28. PMID: 17726021.
      View in: PubMed
    32. Woodworth BA, Wood R, Baatz JE, Schlosser RJ. Sinonasal surfactant protein A1, A2, and D gene expression in cystic fibrosis: a preliminary report. Otolaryngol Head Neck Surg. 2007 Jul; 137(1):34-8. PMID: 17599561.
      View in: PubMed
    33. Woodworth BA, Wood R, Bhargave G, Cohen NA, Baatz JE, Schlosser RJ. Surfactant protein B detection and gene expression in chronic rhinosinusitis. Laryngoscope. 2007 Jul; 117(7):1296-301. PMID: 17507829.
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    34. Woodworth BA, Neal JG, Newton D, Joseph K, Kaplan AP, Baatz JE, Schlosser RJ. Surfactant protein A and D in human sinus mucosa: a preliminary report. ORL J Otorhinolaryngol Relat Spec. 2007; 69(1):57-60. PMID: 17085954.
      View in: PubMed
    35. Tsuruga E, Rao DS, Baatz JE, Reddy SV. Elevated serum kininogen in patients with Paget's disease of bone: a role in marrow stromal/preosteoblast cell proliferation. J Cell Biochem. 2006 Aug 15; 98(6):1681-8. PMID: 16598774.
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    36. Newton DA, Rao KM, Dluhy RA, Baatz JE. Hemoglobin is expressed by alveolar epithelial cells. J Biol Chem. 2006 Mar 03; 281(9):5668-76. PMID: 16407281.
      View in: PubMed
    37. Yu J, Kindy MS, Ellis BC, Baatz JE, Peden-Adams M, Ellingham TJ, Wolff DJ, Fair PA, Gattoni-Celli S. Establishment of epidermal cell lines derived from the skin of the Atlantic bottlenose dolphin (Tursiops truncatus). Anat Rec A Discov Mol Cell Evol Biol. 2005 Dec; 287(2):1246-55. PMID: 16281302.
      View in: PubMed
    38. Chang Y, Wang Z, Schwan AL, Wang Z, Holm BA, Baatz JE, Notter RH. Surface properties of sulfur- and ether-linked phosphonolipids with and without purified hydrophobic lung surfactant proteins. Chem Phys Lipids. 2005 Oct; 137(1-2):77-93. PMID: 16109391.
      View in: PubMed
    39. Wagner CL, Baatz JE. Higher molecular mass forms of TGFalpha in human milk. Adv Exp Med Biol. 2004; 554:411-5. PMID: 15384613.
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    40. Wagner CL, Baatz JE. TGFalpha within compartments of human milk. Adv Exp Med Biol. 2004; 554:417-21. PMID: 15384614.
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    41. Dluhy RA, Shanmukh S, Leapard JB, Krüger P, Baatz JE. Deacylated pulmonary surfactant protein SP-C transforms from alpha-helical to amyloid fibril structure via a pH-dependent mechanism: an infrared structural investigation. Biophys J. 2003 Oct; 85(4):2417-29. PMID: 14507705.
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    42. Acierno PM, Newton DA, Brown EA, Maes LA, Baatz JE, Gattoni-Celli S. Cross-reactivity between HLA-A2-restricted FLU-M1:58-66 and HIV p17 GAG:77-85 epitopes in HIV-infected and uninfected individuals. J Transl Med. 2003 Aug 14; 1(1):3. PMID: 14527342.
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    43. Krüger P, Baatz JE, Dluhy RA, Lösche M. Effect of hydrophobic surfactant protein SP-C on binary phospholipid monolayers. Molecular machinery at the air/water interface. Biophys Chem. 2002 Nov 06; 99(3):209-28. PMID: 12408937.
      View in: PubMed
    44. Wang Z, Baatz JE, Holm BA, Notter RH. Content-dependent activity of lung surfactant protein B in mixtures with lipids. Am J Physiol Lung Cell Mol Physiol. 2002 Nov; 283(5):L897-906. PMID: 12376341.
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    45. Shanmukh S, Howell P, Baatz JE, Dluhy RA. Effect of hydrophobic surfactant proteins SP-B and SP-C on phospholipid monolayers. Protein structure studied using 2D IR and beta correlation analysis. Biophys J. 2002 Oct; 83(4):2126-41. PMID: 12324430.
      View in: PubMed
    46. Geerling G, Baatz J, Harder D, Müller G, Reimer K, Rudolph P, Kramer A. Requirements concerning antiseptics for periorbital, orbital and intraorbital application. Local tolerance. Dev Ophthalmol. 2002; 33:32-56. PMID: 12236124.
      View in: PubMed
    47. Baatz JE, Zou Y, Cox JT, Wang Z, Notter RH. High-yield purification of lung surfactant proteins sp-b and sp-c and the effects on surface activity. Protein Expr Purif. 2001 Oct; 23(1):180-90. PMID: 11570861.
      View in: PubMed
    48. Baatz JE, Zou Y, Korfhagen TR. Inhibitory effects of tumor necrosis factor-alpha on cationic lipid-mediated gene delivery to airway cells in vitro. Biochim Biophys Acta. 2001 Feb 14; 1535(2):100-9. PMID: 11341998.
      View in: PubMed
    49. Tokieda K, Ikegami M, Wert SE, Baatz JE, Zou Y, Whitsett JA. Surfactant protein B corrects oxygen-induced pulmonary dysfunction in heterozygous surfactant protein B-deficient mice. Pediatr Res. 1999 Dec; 46(6):708-14. PMID: 10590028.
      View in: PubMed
    50. Miles PR, Bowman L, Rao KM, Baatz JE, Huffman L. Pulmonary surfactant inhibits LPS-induced nitric oxide production by alveolar macrophages. Am J Physiol. 1999 01; 276(1):L186-96. PMID: 9887071.
      View in: PubMed
    51. Horowitz AD, Moussavian B, Han ED, Baatz JE, Whitsett JA. Distinct effects of SP-A and SP-B on endocytosis of SP-C by pulmonary epithelial cells. Am J Physiol. 1997 Jul; 273(1 Pt 1):L159-71. PMID: 9252553.
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    52. Wang Z, Gurel O, Baatz JE, Notter RH. Acylation of pulmonary surfactant protein-C is required for its optimal surface active interactions with phospholipids. J Biol Chem. 1996 Aug 09; 271(32):19104-9. PMID: 8702584.
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    53. Wang Z, Gurel O, Baatz JE, Notter RH. Differential activity and lack of synergy of lung surfactant proteins SP-B and SP-C in interactions with phospholipids. J Lipid Res. 1996 Aug; 37(8):1749-60. PMID: 8864959.
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    54. Ross GF, Morris RE, Ciraolo G, Huelsman K, Bruno M, Whitsett JA, Baatz JE, Korfhagen TR. Surfactant protein A-polylysine conjugates for delivery of DNA to airway cells in culture. Hum Gene Ther. 1995 Jan; 6(1):31-40. PMID: 7703285.
      View in: PubMed
    55. Baatz JE, Bruno MD, Ciraolo PJ, Glasser SW, Stripp BR, Smyth KL, Korfhagen TR. Utilization of modified surfactant-associated protein B for delivery of DNA to airway cells in culture. Proc Natl Acad Sci U S A. 1994 Mar 29; 91(7):2547-51. PMID: 8146151.
      View in: PubMed
    56. Horowitz AD, Baatz JE, Whitsett JA. Lipid effects on aggregation of pulmonary surfactant protein SP-C studied by fluorescence energy transfer. Biochemistry. 1993 Sep 21; 32(37):9513-23. PMID: 8373758.
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    57. Baatz JE, Smyth KL, Whitsett JA, Baxter C, Absolom DR. Structure and functions of a dimeric form of surfactant protein SP-C: a Fourier transform infrared and surfactometry study. Chem Phys Lipids. 1992 Nov; 63(1-2):91-104. PMID: 1486663.
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    58. Horowitz AD, Elledge B, Whitsett JA, Baatz JE. Effects of lung surfactant proteolipid SP-C on the organization of model membrane lipids: a fluorescence study. Biochim Biophys Acta. 1992 Jun 11; 1107(1):44-54. PMID: 1616924.
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    59. Baatz JE, Sarin V, Absolom DR, Baxter C, Whitsett JA. Effects of surfactant-associated protein SP-B synthetic analogs on the structure and surface activity of model membrane bilayers. Chem Phys Lipids. 1991 Dec; 60(2):163-78. PMID: 1814640.
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    60. Venkitaraman AR, Baatz JE, Whitsett JA, Hall SB, Notter RH. Biophysical inhibition of synthetic phospholipid-lung surfactant apoprotein admixtures by plasma proteins. Chem Phys Lipids. 1991 Jan-Feb; 57(1):49-57. PMID: 2060063.
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    61. Baatz JE, Elledge B, Whitsett JA. Surfactant protein SP-B induces ordering at the surface of model membrane bilayers. Biochemistry. 1990 Jul 17; 29(28):6714-20. PMID: 2397209.
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    62. Rice WR, Sarin VK, Fox JL, Baatz J, Wert S, Whitsett JA. Surfactant peptides stimulate uptake of phosphatidylcholine by isolated cells. Biochim Biophys Acta. 1989 Nov 28; 1006(2):237-45. PMID: 2597670.
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    63. Glaser TA, Baatz JE, Kreishman GP, Mukkada AJ. pH homeostasis in Leishmania donovani amastigotes and promastigotes. Proc Natl Acad Sci U S A. 1988 Oct; 85(20):7602-6. PMID: 3174653.
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