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Edward L. Krug

Title
InstitutionMedical University of South Carolina
DepartmentRegenerative Medicine and Cell Biology
AddressP.O. Box MSC 501
BE 101E
Bioengineering Bldg - 68 President St.
Phone843-876-2404
Fax843-876-2416
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    I was trained in a wide variety of technologies as a protein biochemist in graduate school, which served as the foundation of my postdoctoral work on identifying extracellular signals that induce transformation events in early heart morphogenesis. By using complementary protein and molecular approaches we were able to identify trace levels of an extracellular aggregate of proteins, what are now known to be secretory vesicles termed “exosomes”, that induce the formation of valvuloseptal precursors in the embryonic heart. As part of an NIH funded Cardiovascular Proteomics Center our group developed novel technologies for characterizing dynamics of the embryonic heart proteome. This allowed us to use a platform of methodologies with complementary strengths, e.g., 2D difference-in-gel electrophoresis (DIGE) and the MS-MS based method of isobaric tag for relative and absolute quantitation (iTRAQ) to compare posttranslational modifications and differential composition of protein extracts, and MALDI-TOF imaging of tissue sections to discover spatial changes in protein distribution. We also developed a bioinformatics tool for staging embryonic hearts based on their MALDI-TOF profiles via a Random Forest algorithm. Current projects involve using these approaches to assess subtle changes in the proteome that precede overt morphological defects in the developing heart and cochlea in response to potential teratogens, gene knockout models and aging.

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    Early heart morphogenesis, Biomedical career development, Research integrity

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    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
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    1. Noble KV, Reyzer ML, Barth JL, McDonald H, Tuck M, Schey KL, Krug EL, Lang H. Use of Proteomic Imaging Coupled With Transcriptomic Analysis to Identify Biomolecules Responsive to Cochlear Injury. Front Mol Neurosci. 2018; 11:243. PMID: 30065626.
      View in: PubMed
    2. Moreno-Rodriguez RA, Krug EL, Reyes L, Markwald RR. Linear array of multi-substrate tracts for simultaneous assessment of cell adhesion, migration, and differentiation. Biotechniques. 2017 12 01; 63(6):267-274. PMID: 29235973.
      View in: PubMed
    3. Lang H, Xing Y, Brown LN, Samuvel DJ, Panganiban CH, Havens LT, Balasubramanian S, Wegner M, Krug EL, Barth JL. Neural stem/progenitor cell properties of glial cells in the adult mouse auditory nerve. Sci Rep. 2015 Aug 26; 5:13383. PMID: 26307538.
      View in: PubMed
    4. Burns TA, Dours-Zimmermann MT, Zimmermann DR, Krug EL, Comte-Walters S, Reyes L, Davis MA, Schey KL, Schwacke JH, Kern CB, Mjaatvedt CH. Imbalanced expression of Vcan mRNA splice form proteins alters heart morphology and cellular protein profiles. PLoS One. 2014; 9(2):e89133. PMID: 24586547.
      View in: PubMed
    5. Lang H, Li M, Kilpatrick LA, Zhu J, Samuvel DJ, Krug EL, Goddard JC. Sox2 up-regulation and glial cell proliferation following degeneration of spiral ganglion neurons in the adult mouse inner ear. J Assoc Res Otolaryngol. 2011 Apr; 12(2):151-71. PMID: 21061038.
      View in: PubMed
    6. Grey AC, Gelasco AK, Section J, Moreno-Rodriguez RA, Krug EL, Schey KL. Molecular morphology of the chick heart visualized by MALDI imaging mass spectrometry. Anat Rec (Hoboken). 2010 May; 293(5):821-8. PMID: 20186963.
      View in: PubMed
    7. Schwacke JH, Hill EG, Krug EL, Comte-Walters S, Schey KL. iQuantitator: a tool for protein expression inference using iTRAQ. BMC Bioinformatics. 2009 Oct 18; 10:342. PMID: 19835628.
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    8. Halushka PV, Krug EL. Is the training of biomedical scientists at a crossroads? Acad Med. 2009 Apr; 84(4):421-3. PMID: 19318769.
      View in: PubMed
    9. Mishima N, Hoffman S, Hill EG, Krug EL. Chick embryos exposed to trichloroethylene in an ex ovo culture model show selective defects in early endocardial cushion tissue formation. Birth Defects Res A Clin Mol Teratol. 2006 Jul; 76(7):517-27. PMID: 16933305.
      View in: PubMed
    10. Moreno-Rodriguez RA, Krug EL, Reyes L, Villavicencio L, Mjaatvedt CH, Markwald RR. Bidirectional fusion of the heart-forming fields in the developing chick embryo. Dev Dyn. 2006 Jan; 235(1):191-202. PMID: 16252277.
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    11. Kern CB, Hoffman S, Moreno R, Damon BJ, Norris RA, Krug EL, Markwald RR, Mjaatvedt CH. Immunolocalization of chick periostin protein in the developing heart. Anat Rec A Discov Mol Cell Evol Biol. 2005 May; 284(1):415-23. PMID: 15803479.
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    12. Almeida JS, Stanislaus R, Krug E, Arthur JM. Normalization and analysis of residual variation in two-dimensional gel electrophoresis for quantitative differential proteomics. Proteomics. 2005 Apr; 5(5):1242-9. PMID: 15732138.
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    13. Kruzynska-Frejtag A, Wang J, Maeda M, Rogers R, Krug E, Hoffman S, Markwald RR, Conway SJ. Periostin is expressed within the developing teeth at the sites of epithelial-mesenchymal interaction. Dev Dyn. 2004 Apr; 229(4):857-68. PMID: 15042709.
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    14. de la Cruz MV, Markwald RR, Krug EL, Rumenoff L, Sánchez Gómez C, Sadowinski S, Galicia TD, Gómez F, Salazar García M, Villavicencio Guzman L, Reyes Angeles L, Moreno-Rodriguez RA. Living morphogenesis of the ventricles and congenital pathology of their component parts. Cardiol Young. 2001 Nov; 11(6):588-600. PMID: 11813909.
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    15. Bianchi LM, Liu H, Krug EL, Capehart AA. Selective and transient expression of a native chondroitin sulfate epitope in Deiters' cells, pillar cells, and the developing tectorial membrane. Anat Rec. 1999 09 01; 256(1):64-71. PMID: 10456986.
      View in: PubMed
    16. Capehart AA, Mjaatvedt CH, Hoffman S, Krug EL. Dynamic expression of a native chondroitin sulfate epitope reveals microheterogeneity of extracellular matrix organization in the embryonic chick heart. Anat Rec. 1999 02 01; 254(2):181-95. PMID: 9972803.
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    17. Nakajima Y, Yamagishi T, Nakamura H, Markwald RR, Krug EL. An autocrine function for transforming growth factor beta 3 in the atrioventricular endocardial cushion tissue formation during chick heart development. Ann N Y Acad Sci. 1998 Oct 23; 857:272-5. PMID: 9917855.
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    18. Nakajima Y, Yamagishi T, Nakamura H, Markwald RR, Krug EL. An autocrine function for transforming growth factor (TGF)-beta3 in the transformation of atrioventricular canal endocardium into mesenchyme during chick heart development. Dev Biol. 1998 Feb 01; 194(1):99-113. PMID: 9473335.
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    19. Capehart AA, Wienecke MM, Kitten GT, Solursh M, Krug EL. Production of a monoclonal antibody by in vitro immunization that recognizes a native chondroitin sulfate epitope in the embryonic chick limb and heart. J Histochem Cytochem. 1997 Nov; 45(11):1567-81. PMID: 9358858.
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    20. Moreno-Rodriguez RA, de la Cruz MV, Krug EL. Temporal and spatial asymmetries in the initial distribution of mesenchyme cells in the atrioventricular canal cushions of the developing chick heart. Anat Rec. 1997 05; 248(1):84-92. PMID: 9143671.
      View in: PubMed
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