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R. Amanda C. Larue

Title
InstitutionMedical University of South Carolina
DepartmentPathology and Laboratory Medicine
AddressP.O. Box MSC 908
432
114 Doughty St.
Phone843-789-6713
Fax843-876-5381
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    Collapse Biography 
    Collapse education and training
    Wofford College, Spartanburg, SCBA, BS05/1997Biology and French
    Medical University of South Carolina, Charleston, SCPhD05/2003Molecular, Cellular, and Pathobiology

    Collapse Overview 
    Collapse overview
    Research efforts in my laboratory are focused on the physio-pathological potential of hematopoietic stem cells (HSCs). The adult bone marrow is thought to contain at least two discernible stem cell populations: HSCs, defined by their capability of hematopoietic reconstitution in vivo, and mesenchymal stromal cells (MSC), originally defined by their in vitro potential to differentiate into mesenchymal tissue cells including fibroblasts, fat, cartilage, and bone. Recent studies suggest that organs once thought to be incapable of regenerative ability may indeed be capable of regeneration and repair via stem cells. While the potential of stem cells is clear from studies in which unfractionated or unpurified populations of bone marrow are transplanted, due to technical challenges, controversies have resulted when attempts are made to assign specific potentials to HSCs or MSCs. This has led to the need for stringent, well-defined lineage tracing methods. To this end, my laboratory has developed a transplantation model in which the bone marrow of lethally irradiated recipient mice is reconstituted by a clonal population of cells derived from a single HSC that expresses enhanced green fluorescent protein. This transplantation model is technically complex and experimentally challenging, with only a few laboratories worldwide capable of executing such a transplantation scheme. Our work also employs novel transgenic models to discern HSC-derived cells in tissues. With an eye towards translational potential, we also employ xenograft models to investigate the role(s) of human HSCs. The development of these tools has allowed my laboratory, my mentees, and my collaborators to conduct paradigm-shifting studies of the roles of HSCs in health and disease. Together, these approaches have resulted in discovery of novel roles for HSC and HSC-derived cells in a variety of pathological conditions that directly impact human health, including cancer, cardiovascular disease, pulmonary fibrosis, and orthopedic injury and repair.
    Collapse keywords
    Stem cell, Hematopoietic stem cell, mesenchymal stem cell, immunology, pathology, cancer, orthopedic, pulmonary, cardiovascular, PTSD

    Collapse ORNG Applications 
    Collapse studies/trials

    Collapse Bibliographic 
    Collapse selected publications
    Publications listed below are automatically derived from MEDLINE/PubMed and other sources, which might result in incorrect or missing publications. Faculty can login to make corrections and additions.
    List All   |   Timeline
    1. Durham EL, Balog C, Howie RN, Boyce MA, Arand JR, Warren G, LaRue AC, Cray JJ. Effects of nicotine exposure on murine mandibular development. PLoS One. 2019; 14(6):e0218376. PMID: 31194840.
      View in: PubMed
    2. McDonald LT, Lopez MF, Helke KL, McCrackin MA, Cray JJ, Becker HC, LaRue AC. Early Blood Profile of C57BL/6 Mice Exposed to Chronic Unpredictable Stress. Front Psychiatry. 2019; 10:230. PMID: 31068843.
      View in: PubMed
    3. Kelly RR, Mccrackin MA, Russell DL, Leddy LR, Cray JJ, Larue AC. Teaching Surgical Model Development in Research by Using Situated Learning and Instructional Scaffolding. J Am Assoc Lab Anim Sci. 2019 05 01; 58(3):321-328. PMID: 30987688.
      View in: PubMed
    4. Kelly RR, McDonald LT, Jensen NR, Sidles SJ, LaRue AC. Impacts of Psychological Stress on Osteoporosis: Clinical Implications and Treatment Interactions. Front Psychiatry. 2019; 10:200. PMID: 31024360.
      View in: PubMed
    5. Brinton DL, Simpson AN, Fominaya CE, LaRue AC. Impact of selective serotonin reuptake inhibitors in the veteran population: 10-year risk outcomes. J Comp Eff Res. 2019 Apr; 8(6):431-440. PMID: 30855179.
      View in: PubMed
    6. Grey ZJ, Howie RN, Durham EL, Hall SR, Helke KL, Steed MB, LaRue AC, Muise-Helmericks RC, Cray JJ. Sub-clinical dose of bone morphogenetic protein-2 does not precipitate rampant, sustained inflammatory response in bone wound healing. Wound Repair Regen. 2019 07; 27(4):335-344. PMID: 30805987.
      View in: PubMed
    7. Sidles SJ, Xiong Y, Young MRI, LaRue AC. High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45+DDR2+ Cells. Mediators Inflamm. 2019; 2019:1648614. PMID: 31015794.
      View in: PubMed
    8. Sun B, Jensen NR, Chung D, Yang M, LaRue AC, Cheung HW, Wang Q. Synergistic effects of SHP2 and PI3K pathway inhibitors in GAB2-overexpressing ovarian cancer. Am J Cancer Res. 2019; 9(1):145-159. PMID: 30755818.
      View in: PubMed
    9. Durham EL, Howie RN, Hall S, Larson N, Oakes B, Houck R, Grey Z, Steed M, LaRue AC, Muise-Helmericks R, Cray J. Optimizing bone wound healing using BMP2 with absorbable collagen sponge and Talymed nanofiber scaffold. J Transl Med. 2018 11 21; 16(1):321. PMID: 30463618.
      View in: PubMed
    10. Kelly RR, McDonald LT, Pellegrini VD, Cray JJ, Larue AC. Identification of circulating murine CD34+OCN+ cells. Cytotherapy. 2018 11; 20(11):1371-1380. PMID: 30340982.
      View in: PubMed
    11. Carrell RK, Stanton RA, Ethier SP, LaRue AC, Soloff AC. ICOSL-augmented adenoviral-based vaccination induces a bipolar Th17/Th1 T cell response against unglycosylated MUC1 antigen. Vaccine. 2018 10 08; 36(42):6262-6269. PMID: 30219366.
      View in: PubMed
    12. Howie RN, Herberg S, Durham E, Grey Z, Bennfors G, Elsalanty M, LaRue AC, Hill WD, Cray JJ. Selective serotonin re-uptake inhibitor sertraline inhibits bone healing in a calvarial defect model. Int J Oral Sci. 2018 09 03; 10(3):25. PMID: 30174329.
      View in: PubMed
    13. Wilson KR, Kang IH, Baliga U, Xiong Y, Chatterjee S, Moore E, Parthiban B, Thyagarajan K, Borke JL, Mehrotra S, Kirkwood KL, LaRue AC, Ogawa M, Mehrotra M. Hematopoietic Stem Cells as a Novel Source of Dental Tissue Cells. Sci Rep. 2018 05 23; 8(1):8026. PMID: 29795229.
      View in: PubMed
    14. Kimbrough D, Wang SH, Wright LH, Mani SK, Kasiganesan H, LaRue AC, Cheng Q, Nadig SN, Atkinson C, Menick DR. HDAC inhibition helps post-MI healing by modulating macrophage polarization. J Mol Cell Cardiol. 2018 06; 119:51-63. PMID: 29680681.
      View in: PubMed
    15. McDonald LT, Zile MR, Zhang Y, Van Laer AO, Baicu CF, Stroud RE, Jones JA, LaRue AC, Bradshaw AD. Increased macrophage-derived SPARC precedes collagen deposition in myocardial fibrosis. Am J Physiol Heart Circ Physiol. 2018 07 01; 315(1):H92-H100. PMID: 29522370.
      View in: PubMed
    16. McDonald LT, Johnson SD, Russell DL, Young MRI, LaRue AC. Role of a novel immune modulating DDR2-expressing population in silica-induced pulmonary fibrosis. PLoS One. 2017; 12(7):e0180724. PMID: 28700752.
      View in: PubMed
    17. Xiong Y, Russell DL, McDonald LT, Cowart LA, LaRue AC. Hematopoietic Stem Cell-derived Adipocytes Promote Tumor Growth and Cancer Cell Migration. Int J Cancer Res Mol Mech. 2017; 3(1). PMID: 28989976.
      View in: PubMed
    18. Evans-Knowell A, LaRue AC, Findlay VJ. MicroRNAs and Their Impact on Breast Cancer, the Tumor Microenvironment, and Disparities. Adv Cancer Res. 2017; 133:51-76. PMID: 28052821.
      View in: PubMed
    19. Lang H, Nishimoto E, Xing Y, Brown LN, Noble KV, Barth JL, LaRue AC, Ando K, Schulte BA. Contributions of Mouse and Human Hematopoietic Cells to Remodeling of the Adult Auditory Nerve After Neuron Loss. Mol Ther. 2016 Nov; 24(11):2000-2011. PMID: 27600399.
      View in: PubMed
    20. An N, Xiong Y, LaRue AC, Kraft AS, Cen B. Activation of Pim Kinases Is Sufficient to Promote Resistance to MET Small-Molecule Inhibitors. Cancer Res. 2015 Dec 15; 75(24):5318-28. PMID: 26670562.
      View in: PubMed
    21. Ogawa M, LaRue AC, Mehrotra M. Plasticity of hematopoietic stem cells. Best Pract Res Clin Haematol. 2015 Jun-Sep; 28(2-3):73-80. PMID: 26590762.
      View in: PubMed
    22. McDonald LT, LaRue AC. Bone Marrow Stem Cell Contribution to Pulmonary Homeostasis and Disease. J Bone Marrow Res. 2015 Sep; 3(3). PMID: 26798846.
      View in: PubMed
    23. Yang A, Xiao X, Zhao M, LaRue AC, Schulte BA, Wang GY. Differential Reponses of Hematopoietic Stem and Progenitor Cells to mTOR Inhibition. Stem Cells Int. 2015; 2015:561404. PMID: 26221145.
      View in: PubMed
    24. McDonald LT, Mehrotra M, LaRue AC. Hematopoietic Origin of Murine Lung Fibroblasts. Stem Cells Int. 2015; 2015:159713. PMID: 26185498.
      View in: PubMed
    25. McDonald LT, Russell DL, Kelly RR, Xiong Y, Motamarry A, Patel RK, Jones JA, Watson PM, Turner DP, Watson DK, Soloff AC, Findlay VJ, LaRue AC. Hematopoietic stem cell-derived cancer-associated fibroblasts are novel contributors to the pro-tumorigenic microenvironment. Neoplasia. 2015 May; 17(5):434-48. PMID: 26025666.
      View in: PubMed
    26. Xiong Y, McDonald LT, Russell DL, Kelly RR, Wilson KR, Mehrotra M, Soloff AC, LaRue AC. Hematopoietic stem cell-derived adipocytes and fibroblasts in the tumor microenvironment. World J Stem Cells. 2015 Mar 26; 7(2):253-65. PMID: 25815113.
      View in: PubMed
    27. Xiao X, Luo H, Vanek KN, LaRue AC, Schulte BA, Wang GY. Catalase inhibits ionizing radiation-induced apoptosis in hematopoietic stem and progenitor cells. Stem Cells Dev. 2015 Jun 01; 24(11):1342-51. PMID: 25603016.
      View in: PubMed
    28. Cen B, Xiong Y, Song JH, Mahajan S, DuPont R, McEachern K, DeAngelo DJ, Cortes JE, Minden MD, Ebens A, Mims A, LaRue AC, Kraft AS. The Pim-1 protein kinase is an important regulator of MET receptor tyrosine kinase levels and signaling. Mol Cell Biol. 2014 Jul; 34(13):2517-32. PMID: 24777602.
      View in: PubMed
    29. Ogawa M, LaRue AC, Mehrotra M. Hematopoietic stem cells are pluripotent and not just "hematopoietic". Blood Cells Mol Dis. 2013 Jun; 51(1):3-8. PMID: 23453528.
      View in: PubMed
    30. Guo QJ, Mills JN, Bandurraga SG, Nogueira LM, Mason NJ, Camp ER, Larue AC, Turner DP, Findlay VJ. MicroRNA-510 promotes cell and tumor growth by targeting peroxiredoxin1 in breast cancer. Breast Cancer Res. 2013; 15(4):R70. PMID: 23971998.
      View in: PubMed
    31. Findlay VJ, LaRue AC, Turner DP, Watson PM, Watson DK. Understanding the role of ETS-mediated gene regulation in complex biological processes. Adv Cancer Res. 2013; 119:1-61. PMID: 23870508.
      View in: PubMed
    32. McDonald LT, LaRue AC. Hematopoietic stem cell derived carcinoma-associated fibroblasts: a novel origin. Int J Clin Exp Pathol. 2012; 5(9):863-73. PMID: 23119103.
      View in: PubMed
    33. Mehrotra M, Williams CR, Ogawa M, LaRue AC. Hematopoietic stem cells give rise to osteo-chondrogenic cells. Blood Cells Mol Dis. 2013 Jan; 50(1):41-9. PMID: 22954476.
      View in: PubMed
    34. Turner DP, Findlay VJ, Moussa O, Semenchenko VI, Watson PM, LaRue AC, Desouki MM, Fraig M, Watson DK. Mechanisms and functional consequences of PDEF protein expression loss during prostate cancer progression. Prostate. 2011 Dec; 71(16):1723-35. PMID: 21446014.
      View in: PubMed
    35. Findlay VJ, Turner DP, Yordy JS, McCarragher B, Shriver MR, Szalai G, Watson PM, Larue AC, Moussa O, Watson DK. Prostate-Derived ETS Factor Regulates Epithelial-to-Mesenchymal Transition through Both SLUG-Dependent and Independent Mechanisms. Genes Cancer. 2011 Feb; 2(2):120-9. PMID: 21779485.
      View in: PubMed
    36. Moussa O, LaRue AC, Abangan RS, Williams CR, Zhang XK, Masuya M, Gong YZ, Spyropoulos DD, Ogawa M, Gilkeson G, Watson DK. Thrombocytopenia in mice lacking the carboxy-terminal regulatory domain of the Ets transcription factor Fli1. Mol Cell Biol. 2010 Nov; 30(21):5194-206. PMID: 20823267.
      View in: PubMed
    37. Jyothi V, Li M, Kilpatrick LA, Smythe N, LaRue AC, Zhou D, Schulte BA, Schmiedt RA, Lang H. Unmyelinated auditory type I spiral ganglion neurons in congenic Ly5.1 mice. J Comp Neurol. 2010 Aug 15; 518(16):3254-71. PMID: 20575058.
      View in: PubMed
    38. Mehrotra M, Rosol M, Ogawa M, Larue AC. Amelioration of a mouse model of osteogenesis imperfecta with hematopoietic stem cell transplantation: microcomputed tomography studies. Exp Hematol. 2010 Jul; 38(7):593-602. PMID: 20417683.
      View in: PubMed
    39. Ogawa M, Larue AC, Watson PM, Watson DK. Hematopoietic stem cell origin of connective tissues. Exp Hematol. 2010 Jul; 38(7):540-7. PMID: 20412832.
      View in: PubMed
    40. Ogawa M, Larue AC, Watson PM, Watson DK. Hematopoietic stem cell origin of mesenchymal cells: opportunity for novel therapeutic approaches. Int J Hematol. 2010 Apr; 91(3):353-9. PMID: 20336396.
      View in: PubMed
    41. Abangan RS, Williams CR, Mehrotra M, Duncan JD, Larue AC. MCP1 directs trafficking of hematopoietic stem cell-derived fibroblast precursors in solid tumor. Am J Pathol. 2010 Apr; 176(4):1914-26. PMID: 20167869.
      View in: PubMed
    42. Shirai K, Sera Y, Bulkeley W, Mehrotra M, Moussa O, LaRue AC, Watson DK, Stuart RK, Lazarchick J, Ogawa M. Hematopoietic stem cell origin of human fibroblasts: cell culture studies of female recipients of gender-mismatched stem cell transplantation and patients with chronic myelogenous leukemia. Exp Hematol. 2009 Dec; 37(12):1464-71. PMID: 19786066.
      View in: PubMed
    43. Sera Y, LaRue AC, Moussa O, Mehrotra M, Duncan JD, Williams CR, Nishimoto E, Schulte BA, Watson PM, Watson DK, Ogawa M. Hematopoietic stem cell origin of adipocytes. Exp Hematol. 2009 Sep; 37(9):1108-20, 1120.e1-4. PMID: 19576951.
      View in: PubMed
    44. Ogawa M, LaRue AC. Origin of fibroblast colony-forming units. Exp Hematol. 2007 Sep; 35(9):1319-20. PMID: 17637495.
      View in: PubMed
    45. Szalai G, LaRue AC, Watson DK. Molecular mechanisms of megakaryopoiesis. Cell Mol Life Sci. 2006 Nov; 63(21):2460-76. PMID: 16909203.
      View in: PubMed
    46. Ogawa M, LaRue AC, Drake CJ. Hematopoietic origin of fibroblasts/myofibroblasts: Its pathophysiologic implications. Blood. 2006 Nov 01; 108(9):2893-6. PMID: 16840726.
      View in: PubMed
    47. Visconti RP, Ebihara Y, LaRue AC, Fleming PA, McQuinn TC, Masuya M, Minamiguchi H, Markwald RR, Ogawa M, Drake CJ. An in vivo analysis of hematopoietic stem cell potential: hematopoietic origin of cardiac valve interstitial cells. Circ Res. 2006 Mar 17; 98(5):690-6. PMID: 16456103.
      View in: PubMed
    48. Ebihara Y, Masuya M, Larue AC, Fleming PA, Visconti RP, Minamiguchi H, Drake CJ, Ogawa M. Hematopoietic origins of fibroblasts: II. In vitro studies of fibroblasts, CFU-F, and fibrocytes. Exp Hematol. 2006 Feb; 34(2):219-29. PMID: 16459190.
      View in: PubMed
    49. LaRue AC, Masuya M, Ebihara Y, Fleming PA, Visconti RP, Minamiguchi H, Ogawa M, Drake CJ. Hematopoietic origins of fibroblasts: I. In vivo studies of fibroblasts associated with solid tumors. Exp Hematol. 2006 Feb; 34(2):208-18. PMID: 16459189.
      View in: PubMed
    50. Wang Y, Schulte BA, LaRue AC, Ogawa M, Zhou D. Total body irradiation selectively induces murine hematopoietic stem cell senescence. Blood. 2006 Jan 01; 107(1):358-66. PMID: 16150936.
      View in: PubMed
    51. LaRue AC, Argraves WS, Zile MH, Drake CJ. Critical role for retinol in the generation/differentiation of angioblasts required for embryonic blood vessel formation. Dev Dyn. 2004 Aug; 230(4):666-74. PMID: 15254901.
      View in: PubMed
    52. Drake CJ, Fleming PA, Larue AC, Barth JL, Chintalapudi MR, Argraves WS. Differential distribution of cubilin and megalin expression in the mouse embryo. Anat Rec A Discov Mol Cell Evol Biol. 2004 Mar; 277(1):163-70. PMID: 14983511.
      View in: PubMed
    53. LaRue AC, Lansford R, Drake CJ. Circulating blood island-derived cells contribute to vasculogenesis in the embryo proper. Dev Biol. 2003 Oct 01; 262(1):162-72. PMID: 14512026.
      View in: PubMed
    54. LaRue AC, Mironov VA, Argraves WS, Czirók A, Fleming PA, Drake CJ. Patterning of embryonic blood vessels. Dev Dyn. 2003 Sep; 228(1):21-9. PMID: 12950076.
      View in: PubMed
    55. Argraves WS, Larue AC, Fleming PA, Drake CJ. VEGF signaling is required for the assembly but not the maintenance of embryonic blood vessels. Dev Dyn. 2002 Nov; 225(3):298-304. PMID: 12412012.
      View in: PubMed
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